Abstract
A carboxyl ion implantation-modified indium tin oxide (COOH/ITO) electrode was prepared, and the existence of a carboxyl group on the electrode was verified by X-ray photoelectron spectroscopy (XPS). Hemoglobin (Hb) was immobilized on the electrode, and cyclic voltammetric techniques were employed to evaluate the electrochemical behavior of Hb. Based on the activity of Hb on the COOH/ITO electrode towards the reduction of hydrogen peroxide, a mediator-free H2O2 biosensor was constructed, which showed a linear range from 1.0 μM to 200.0 μM with a detection limit of 0.3 μM (S/N = 3).
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