Abstract
Direct Dried Stool Sampling on Filter Paper for Molecular Analyses of Cholera.
Highlights
While investigating the origin of a cholera epidemic in the Republic of Guinea[3] in September 2012, we experimented direct dried stool sampling on filter paper in the neighboring Sierra Leone, without prior enrichment in APW
With official authorization of Sierra Leonean health authorities, 17 suspected cholera patients were anonymously sampled in cholera treatment units in several coastal districts (Supplemental Figure 1)
One or two drops of their watery diarrhea were directly collected from nonchlorinated buckets below cholera cots and deposited onto each sample area of Whatman FTA Elute Micro Cards
Summary
While investigating the origin of a cholera epidemic in the Republic of Guinea[3] in September 2012, we experimented direct dried stool sampling on filter paper in the neighboring Sierra Leone, without prior enrichment in APW. With official authorization of Sierra Leonean health authorities, 17 suspected cholera patients were anonymously sampled in cholera treatment units in several coastal districts (Supplemental Figure 1). A 1-cm[2] area of the filter paper was removed and suspended in 500 μL NucliSENS® easyMAG® lysis buffer (bioMérieux, Marcy l’Etoile, France) overnight at 4°C. The DNA was extracted using a NucliSENS® easyMAG® platform (bioMérieux, Marcy l’Etoile, France) as previously described,[7] and the supernatants were stored at À20°C for downstream PCR assays.
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