Direct determination of small cations in proteinaceous samples using a flow injection–capillary electrophoresis system

Abstract

A method is described for the direct determination of small inorganic cations in samples containing large amounts of proteins, such as milk or blood plasma. The method is based on electrokinetic injection in a flow injection analysis–capillary electrophoresis (CE) system. The selected CE-electrolyte, containing 5 m M 4-aminopyridine and 7 μ M cetyltrimethylammonium bromide at pH 4.5, prevents detrimental protein adsorption on the capillary walls. Therefore, no sample pretreatment, except for dilution, is required. Up to 30 repeated injections in one electrophoretic run can be performed, yielding RSD values of the migration time of less than 1 and 2.5% ( n=30) for milk and blood plasma samples, respectively.