Abstract
Many studies on the development of PCR for the detection of resistance genes have already been published, including multiplex PCR methods. However, cross-amplification reactions can be a major concern in multiplex PCR methods. In this study, we developed a highly sensitive and specific multiplex-touchdown PCR assay for simultaneous detection of mecA, blaSHV , blaCTX-M , blaTEM and blaOXA genes from positive blood culture bottles, cross-amplification was absent and false-positive results were not obtained.
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