Abstract
Tetrahymena14-nm filament protein/citrate synthase (49K protein) is a bifunctional protein with roles in the cytoskeleton and as a citrate synthase. Though previous studies have shown that the 49K protein is derived from a single transcript of a single gene, direct demonstration of the 49K protein's bifunctional property remained to be elucidated. In this study, a recombinant 49K protein was expressed inEscherichia coli,purified and characterized. The citrate synthase activity of the recombinant 49K protein was comparable to that of the 49K protein purified fromTetrahymena.The recombinant 49K protein formed 14-nm filaments, but only of short length. The filaments were elongated in the presence of a soluble fraction ofTetrahymena.These results suggest that the 49K protein itself is bifunctional, but some co-factor(s) is necessary for elongation of filaments.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
