Abstract
A reagent kit EIA-OCHRATOXIN A for the determination of mycotoxin ochratoxin A in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The conjugate of ochratoxin A with horseradish peroxidase was synthesized using the mycotoxin aminoderivative joined to the carbohydrate chain of the enzyme. A monoclonal antibody to the mycotoxin was immobilized on the surface of plate wells through sheep anti-mouse antibodies. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of ochratoxin A content of agricultural products in a range of 5 to 375 mg/kg with proper accuracy and precision. The limit of quantitative determination of ochratoxin A in grain and cereal foods does not exceed 5 mg/kg.
Highlights
synthesized using the mycotoxin aminoderivative joined to the carbohydrate chain of the enzyme
A monoclonal antibody to the mycotoxin was immobilized on the surface of plate wells
metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development
Summary
Разработан и испытан набор реагентов «ИФА-ОХРАТОКСИН А» для определения охратоксина А в кормах и пищевой продукции методом прямого конкурентного иммуноферментного анализа в микропланшетном формате. Конъюгат охратоксина А с пероксидазой из корней хрена получен с использованием аминопроизводного микотоксина, присоединенного к углеводной цепи фермента. Установлены технико-аналитические параметры набора «ИФА-ОХРАТОКСИН А» и метрологические характеристики методики выполнения измерений. Набор позволяет с надлежащей точностью определять охратоксин А в диапазоне содержания от 5 до 375 мкг/кг, предел количественного определения микотоксина в зерне и в продуктах его переработки составляет 5 мкг/кг. Ключевые слова: микотоксины, охратоксин А, иммуноферментный анализ Для цитирования. Метод прямого конкурентного иммуноферментного анализа для определения охратоксина А в кормах и пищевых продуктах / И.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings of the National Academy of Sciences of Belarus, Chemical Series
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
