Abstract
Guava (Psidium guajava L.) is one of the important fruit crops which are grown in tropical and subtropical countries including India. Wilt is an important disease in guava which is caused by Fusarium oxysporum f. sp. psidii (Fop) as a major obstacle for guava fruit production. It is manifested symptomatically with alterations in the development process such as premature shedding of leaves, pre-maturation of fruits, entire/whole tree defoliation and eventually death of the plant. In this study a colony PCR assay was developed for direct amplification of Internal Transcribed Spacer (ITS) region of fungal rDNA of Fop isolates. For this, two sets of primers were developed for amplification of ITS region. . The sensitivity of both the primer pair was ranged up to 10-6 and 10-7 dilutions of the pure mycelium suspension. This study provides new insight for rapid, sensitive and specific molecular detection of Fop isolates, and also useful for earlier diagnostic for better management of guava wilt disease.
Highlights
Guava (Psidium guajava L.), an arborescent shrub or a small tree, is one of the very productive and highly profitable fruit crop
A number of reports are available on wilt disease is caused by Fusarium species [1,2,3,4] but the most common fungi associated with wilt disease is F. oxysporum f. sp. psidii [5, 6]
The causal organism has been identified as F. oxysporum f. sp. psidii
Summary
Guava (Psidium guajava L.), an arborescent shrub or a small tree, is one of the very productive and highly profitable fruit crop It belongs to the family myrtaceae and is being grown all over the sub-tropical and tropical regions globally. Polymerase Chain Reaction (PCR) has been widely assessed in the field of mycology for diverse genetic analyses, specific detection, phylogenetic studies for identification and taxonomy studies [9]. These molecular studies require a number of laborious steps, including preparation of a DNA template by extraction of DNA from fungi [10]. Roberto et al [11] reported that the omission of the DNA extraction procedure, which significantly decreases time and cost; and avoids the risk of contamination during the DNA extraction process
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