Abstract
In this study, we demonstrate the one-step production of cadaverine (1,5-diaminopentane) from cellobiose using an Escherichia coli strain displaying β-glucosidase (BGL) on its cell surface. L-lysine decarboxylase (CadA) derived from E. coli and BGL from Thermobifida fusca YX (Tfu0937) fused to the anchor protein Blc from E. coli were co-expressed using E. coli as a host. The expression of CadA was confirmed by Western blotting and BGL activity on the cell surface was evaluated using pNPG as a substrate. Growth on cellobiose as the sole carbon source was also achieved. The OD600 value of the BGL and CadA co-expressing strain was 8.0 after 48 h cultivation, which is higher than that obtained by growth on glucose (5.4 after 48 h cultivation). The engineered strain produced cadaverine from cellobiose more effectively than from glucose: 6.1 mM after 48 h from 28 g/L of consumed cellobiose, vs. 3.3 mM from 20 g/L of consumed glucose.
Highlights
Cadaverine, known as 1,5-diaminopentane, has many industrial applications
The aim of this study was the direct production of cadaverine from cellobiose using E. coli expressing BGL and cell surface. L-lysine decarboxylase (CadA)
We previously reported the direct utilization of cellobiose using E. coli displaying BGL (Tanaka et al 2011)
Summary
Cadaverine, known as 1,5-diaminopentane, has many industrial applications. Similar to putrescine (1,4-diaminobutane), cadaverine serves as a component of polymers such as polyamides and polyurethane, and as a component of chelating agents and other additives. Cadaverine is relevant in the production of bio-polyamides derived from renewable feedstocks as a replacement for conventional polyamides derived from petrochemicals. The petrochemical route suffers from the limited supply and rising price of fossil fuels, as well as from low eco-efficiency. Polyamide 54 is made by the polycondensation of cadaverine and succinic acid. Polyamide 54 holds promise as a bio-based alternative to conventional petroleum-based polyamides that are currently produced on the order of 3.5 million tons annually (Kind et al 2010; Mimitsuka et al 2007)
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