Abstract
The hairy vetch (Vicia villosa Roth) is a climbing, prostrate or trailing legume grown as forage.It fixes atmospheric nitrogen, reduces soil erosion and provides an instant mulch. Multiple axillary shoot regeneration from a mature seed explant (zygotic embryo with two cotyledons) was obtained on MS medium containing 0.05 - 1.6 mg/l TDZ with or without 0.10 mg/l IBA. The frequency (%) of shoot regeneration ranged from 45.83-75.00% with a maximum number of 28.6 shoots per explant on MS medium containing 0.20 mg/l TDZ-0.10 mg/l IBA. The mean shoot length decreased proportionately with each increase in TDZ concentration irrespective of the IBA concentration in the culture medium. However, comparing the two types of regeneration media, longer shoots were recorded in the presence of IBA in the culture medium. Regenerated shoots were pulse treated with 50 mg/l IBA for 5, 10 and 20 min for rooting.
Highlights
The hairy vetch (Vicia villosa), known as fodder vetch or winter vetch, is an important fodder legume grown as a forage crop and as a companion plant with tomato (Abdul-Baki and Teasdale, 1993)
Multiple shoot regeneration without callus induction started from the embryonic axis after three weeks of culture (Fig 2a)
Direct in vitro regeneration of hairy vetch plantlets under in vitro conditions from a mature embryo with two cotyledons explant is an important achievement for this important plant species
Summary
The hairy vetch (Vicia villosa), known as fodder vetch or winter vetch, is an important fodder legume grown as a forage crop and as a companion plant with tomato (Abdul-Baki and Teasdale, 1993). It is native to Europe and western Asia It is a climbing, prostrate or trailing legume and has good tolerance to adverse soils; it can be grown under low or high rainfall conditions in a wide pH range of 4.9 to 8.2 (Duke and James, 1990). Successful in vitro shoot regeneration has been reported in other vetches, such as the narbon vetch (Donn, 1978; Roupakias, 1985; Pickardt and Schieder, 1987; Pickardt et al, 1989; Albrecht and Kohlenbach, 1989; Tegeder et al, 1996; Kendir et al, 2008, 2009), Hungarian vetch (Sancak et al, 2000; SahinDemirbag et al, 2008) and faba bean (Fakhrai et al, 1989; Khalafalla and Kazumi, 1999). To the best of our knowledge, to date there is no report available on in vitro shoot regeneration in the hairy vetch
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