Direct and delayed effects of exposure to a sublethal concentration of the insecticide λ-cyhalothrin on food consumption and reproduction of a leaf beetle

Abstract

Anthropogenic pollution such as the application of pesticides poses a major threat to many (non-target) organisms. However, little is known about the persistence of harmful effects or potential recovery in response to a period of exposure to a sublethal insecticide dose. Adults of the mustard leaf beetle, Phaedon cochleariae (Coleoptera: Chrysomelidae), were either exposed to a sublethal concentration of the pyrethroid λ-cyhalothrin for two weeks or kept unexposed as control. During, immediately after and at a delayed time after the exposure, consumption and reproduction, i.e., number of eggs laid and hatching success, were assessed. In addition, long-term effects on unexposed offspring were investigated. Exposure to λ-cyhalothrin reduced the consumption during the insecticide exposure, but led to compensatory feeding in females at a delayed time after exposure. The reproductive output of females was impaired during and directly after λ-cyhalothrin exposure. At the delayed time point there was no clear evidence for a recovery, as the reproduction of heavier females was still negatively affected, while lighter females showed an enhanced reproduction. Persistent negative effects on unexposed offspring had been found when collected from parents directly after a λ-cyhalothrin exposure period. In contrast, in the present experiment neither negative effects on life-history traits nor on consumption were observed in unexposed offspring derived from parents at the delayed time after λ-cyhalothrin exposure. Moreover, eggs of offspring from insecticide-exposed parents showed a higher hatching success than those of offspring of unexposed parents, which may indicate transgenerational hormesis. Our results highlight that λ-cyhalothrin exposure has persistent negative effects on fitness parameters of the exposed generation. However, offspring may not be harmed if their parents had sufficient time to recover after such an insecticide exposure. Taken together, our study emphasises that the time-course of exposure to this anthropogenic pollution is crucial when determining the consequences on life-history.