Dipeptidyl peptidases in bovine reproductive organs and secretions.

Abstract

Dipeptidyl peptidases (DPP) I-IV were analysed in homogenates of bovine reproductive organs as well as in seminal vesicle secretions and seminal plasma. The presence of various molecular forms of these enzymes was studied by fractionation using gel filtration, anion exchange chromatography and chromatofocusing. The eluting enzymes were pooled, and their biochemical properties were briefly characterized. The histochemical localization of DPP II and IV was carried out with the most active tissues. DPP I and III were absent from seminal plasma, but their highest activity was found in the epididymis and increased during sexual maturation. DPP II was found mainly in a single molecular form and displayed a wide distribution in the reproductive organs. Its activity in seminal plasma may be derived from various organs, although the major sources are probably the apical activity in the epididymis, ampulla and seminal vesicle. DPP IV activity was high in the cauda epididymis, and ampulla, and in the seminal vesicles and their secretions. The high activity of DPP IV in seminal plasma appeared to derive from these organs, which showed a strong apical reaction of the epithelial lining. In seminal vesicles the enzyme was mainly secreted attached to membrane particles called vesiculosomes.