Diosmin protects against acrylamide-induced toxicity in rats: Roles of oxidative stress and inflammation

Abstract

Acrylamide (ACR) is a toxic substance produced by oxidative stress. The recent study focused on stimulation of ACR through ROS intracellular production thereby playing a crucial aspect in the process of toxicity. This is positioned on a relevant research and study on the antioxidant characteristics of diosmin. This research was shown to examine the shielded effect of diosmin against ACR toxicity. Forty rats (male) were grouped in five categories. The control category was given normal saline followed by the second category which was given diosmin (100 mg/kg b.wt oral administered). The next category was given ACR (30 mg/kg b.wt orally) every day for 14 days. The fourth and fifth groups were supplemented by acrylamide (30 mg/kg b.wt) after diosmin given orally (at 50 or 100 mg/kg b.wt for 7days). Upon examination of the specimens over a period of the given time frame, it was found that acrylamide intoxication remarkably rising levels of serum (aspartate transferase, alanine transferase, ALP, urea, creatinine, interleukin 1 beta ,and interleukin 6. Besides, it increased the brain, hepatic and renal lipid peroxidation, at the same time, weakening the antioxidant biomarkers accumulation and activities. The introduction of diosmin also alleviated the serum aspartate transferase, alanine transferase, APL, urea, creatinine, as well as plasma pro-inflammatory cytokines such as interleukin-1-beta and interleukin-6. Further to this, both diosmin doses remarkably lowered the levels of MAD in the tissues and nitric oxide which further increased the glutathione, glutathione peroxidase, superoxide dismutase, and catalase, in contrast to the ACR-injected group. Based on these findings, administered diosmin standardized the modified serum framework, halted the peroxidation, and magnified the accumulation and working effects of the biomarker antioxidants in the brain, hepatic and renal tissues of the rats in the current dose-dependent research. Therefore, these findings show that diosmin has a protective reaction in defiance to the toxicity produced by acrylamide through the free radical scavengers along with potent antioxidant occurrences.