Diopatra cupreaworm burrow parchment: a cautionary tale of infaunal surface reactivity

Abstract

Many infaunal marine invertebrates produce mucus excretions that play an important role in metal binding, authigenic mineralization and burrow stabilization. To date, only a handful of studies have characterized the functional groups that control the surface reactivity of burrow linings and backfills. This makes it difficult to place estimates on the overall impact that bioturbation has on metal cycling in tidal flats, the inner shelf, estuaries and other shallow marine environments. Here, we examined the parchment linings of Diopatra cuprea burrows from the Ogeechee River estuary near Savannah, Georgia, USA. Acid–base titrations coupled with Fourier transform infrared spectroscopy demonstrate that the parchment is essentially composed of hydroxyl (R-OH) groups, yielding total ligand densities of only 0.017 mmol/g. To place this value into context, it is orders of magnitude less than previously reported mucopolysaccharides for other marine worms, indicating that D. cuprea is essentially unreactive in the estuarine waters from which it was collected. This was corroborated by minimal Cd2+ adsorption to, and limited silicification of, pre-rinsed parchment. The lack of silica adsorption was surprising given that the parchment was generally coated with an abundance of quartz grains when extracted from the sediment. This suggests that perhaps the physical, rather than chemical, characteristics of the parchment material were responsible for this association. Indeed, scanning electron microscope images show that the parchment is fibrous and envelopes quartz grains, implying that detritus may get trapped by the parchment mesh. It appears that unlike many other infaunal mucopolysaccharide-rich linings that might be produced to provide reactive surfaces to which dissolved metal cations can adsorb for the organism's nutritional benefit, the parchment of D. cuprea may instead function to protect the animal from stresses such as predation or high-energy disturbances.