Abstract

We report N2 fixation by Klebsiella oxytoca strain NG13, isolated from the rhizosphere of a rice (Oryza sativa L.) plant, in association with Indica type rice strain, C5444. The C2H2‐reducation assays, biomass measurements, N content determinations, and 15N isotopic dilution assays indicated that inoculation of C5444 with NG13 resulted in a 6% increase of total plant+soil N content, and a 19% 15N isotopic dilution of plant N after 120 days of cultivation. Our results demonstrated significant levels of N2 fixation by K. oxytoca when associated with rice. By optimizing such rice plant‐bacterial N2 fixation capacity, the N uptake by rice could be enhance.

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