Abstract
The dinitrogen fixation activity of Azospirillum sp., and Pantoea agglomerans strains was determined by 15N2 incorporation after incubation with 15N2 labeled air or/and by acetylene reduction. These bacterial strains were able to fix N2 both in pure culture and in association with wheat plants in hydroponics. Nitrogenase activity of Azospirillum sp., in pure culture was more rapidly inhibited by the addition of NH4 + than NO3 −. The N2 fixation of P. agglomerans decreased only by NH4 + -addition, but was stimulated by NO3 −. Nitrogen fixation in association with wheat plants remained unaffected by both N compounds. However, nitrogen derived from the atmosphere (Ndfa) contributed only very little to the overall nitrogen nutrition of the plants.
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