Abstract
BackgroundLiver is the primary metabolizing site of body and is prone to damage by exogenous as well as endogenous intoxicants. Polycyclic aromatic hydrocarbons such as 7, 12- dimethylbenz(α)anthracene (DMBA) is an exogenous hepatotoxin, which is well known for modulating phase I, II and anti-oxidative enzymes of liver. Plants contain plethora of polyphenolic compounds which can reverse the damaging effect of various xenobiotics. The present study investigated protective role of the ethyl acetate fraction of Acacia catechu Willd. (EAF) against DMBA induced alteration in hepatic metabolizing and anti-oxidative enzymes in rats.Methodology and Principal FindingsThe rats were subjected to hepatic damage by treating with DMBA for 7 weeks on alternative days and treatment schedule was terminated at the end of 14 weeks. The rats were euthanized at the end of protocol and livers were homogenized. The liver homogenates were used to analyse phase I (NADPH-cytochrome P450 reducatse, NADH-cytochrome b5 reductase, cytochrome P420, cytochrome b5), phase II (glutathione-S-transferase, DT diaphorase and γ-Glutamyl transpeptidase) and antioxidative enzymes (catalase, superoxide dismutase, ascorbate peroxidase, glutathione reductase, guiacol peroxidase and lactate dehydrogenase). Furthermore, other oxidative stress parameters (thiobarbituric acid reactive substances, lipid hydroperoxides and conjugated dienes and reduced glutathione) and liver marker enzymes (serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase and alkaline phosphatase) were also studied. The DMBA induced significant changes in activity of hepatic enzymes that was reversed by treatment with three dose levels of EAF.ConclusionIt is concluded that EAF affords hepato-protection against DMBA in rats through modulation of phase I, II and anti-oxidative enzymes.
Highlights
Liver is the most important organ of body involved in numerous detoxification processes, synthesis of plasma proteins and plays vital role in digestion
It is concluded that ethyl acetate fraction of A catechu (EAF) affords hepato-protection against DMBA in rats through modulation of phase I, II and anti-oxidative enzymes
Enzymes in liver A significant decrease in nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 enzyme activity was observed in liver homogenate of DMBA treated rats as compared to control group
Summary
Liver is the most important organ of body involved in numerous detoxification processes, synthesis of plasma proteins and plays vital role in digestion. Various hepato-toxins act by initiating secondary and tertiary changes in the gene expression, eliciting immune mediated allergic responses and modulating activities of xenobiotic and anti-oxidative enzymes [6]. Polycyclic aromatic hydrocarbons such as 7, 12- dimethylbenz(a)anthracene (DMBA) leads to hepatotoxicity, carcionogenicity as well as alteration of phase I and II enzymes involved in metabolic processes in liver. Liver is the primary metabolizing site of body and is prone to damage by exogenous as well as endogenous intoxicants Polycyclic aromatic hydrocarbons such as 7, 12- dimethylbenz(a)anthracene (DMBA) is an exogenous hepatotoxin, which is well known for modulating phase I, II and anti-oxidative enzymes of liver. The present study investigated protective role of the ethyl acetate fraction of Acacia catechu Willd. (EAF) against DMBA induced alteration in hepatic metabolizing and anti-oxidative enzymes in rats
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