Dimethylethanolamine does not prevent liver failure in phosphatidylethanolamine N-methyltransferase-deficient mice fed a choline-deficient diet

Abstract

Mice that lack phosphatidylethanolamine- N-methyltransferase (PEMT) and are fed a choline-deficient (CD) diet suffer severe liver damage and do not survive. Since phosphatidyldimethylethanolamine (PDME) has physical properties similar to those of phosphatidylcholine (PC), we hypothesized that dimethylethanolamine (DME) would be converted into PDME that might substitute for PC, and therefore abrogate the liver damage in the Pemt −/− mice fed a CD diet. We fed Pemt −/− mice either a CD diet, a CD diet supplemented with choline, or a CD diet supplemented with DME (CD+DME). Pemt −/− mice fed the CD diet developed severe liver failure by 4 days while CD+DME-fed mice developed severe liver failure by 5 days. The hepatic PC level in choline-supplemented (CS) mice was 67±4 nmol/mg protein, whereas the PC content was reduced in CD- and CD+DME-fed mice (49±3 and 30±3 nmol/mg protein, respectively). Upon supplementation of the CD diet with DME the amount of hepatic PDME was 81±9 nmol/mg protein so that the hepatic content of PC+PDME combined was 111 nmol/mg protein. Moreover, plasma apolipoprotein B100 and A1 levels were markedly lower in mice fed the CD+DME diet compared to mice fed the CS diet, as was the plasma content of PC. Thus, despite replacement of the deficit in hepatic PC with PDME in Pemt −/− mice fed a CD diet, normal liver function was not restored. We conclude that although PC and PDME exhibit similar physical properties, the three methyl groups of choline are required for hepatic function in mice.