Abstract
The analyses of toxic and proapop-totic proteins require tightly regulated expression systems, and several systems have been developed to control gene expression in human cells. The tetracycline (Tet)-based regulatory strategies provide the advantage of high expression levels, strong inducibility, and the use of an inducer with well-described pharmacological kinetics and almost no pleiotropic effects in vivo. First developed by Gossen and Bujard in 1992, these are now the most frequently used mammalian inducible expression systems (1,2).The Tet strategies apply elements from the Escherichia coli Tet resis-tance operon to control the expression of a gene of interest. In the Tet-Off
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