Abstract
During our studies on the effects of environmental estrogenic chemicals on vitellogenin gene expression in fish, dimethyl formamide (DMFA) and ethylene glycol (EG) were used as solvents to dissolve and deliver hydrophobic β-estradiol or nonylphenol. Control groups were injected with water only. It was surprising to find that in certain doses, Vg mRNA from control fish can be induced by DMFA or EG alone. This suggested that DMFA and EG are weak estrogens.
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