Abstract
The early diagnosis and monitoring of cancers are key factors in effective cancer treatment. Particularly, the separation of biomolecules is an essential step for both diagnostic and analytical purposes. However, the current techniques used to isolate biomolecules are intensive, laborious, and require multiple instruments as well as repeated sample preparations to separate each biomolecule. Thus, an efficient separation system that can simultaneously separate biomolecules from scarce samples is highly desirable. Hence, in this study, we developed a biosilica-based syringe filtration system for the efficient separation of biomolecules from cancer samples using amine-modified diatomaceous earth (AD) with dimethyl 3,3′-dithiobispropionimidate (DTBP). The syringe filter can be an efficient and rapid tool for use in various procedures without complex instruments. The DTBP-based AD system was combined with the syringe filter system for nucleic acid and protein separation from various cancer cells. We demonstrated the efficacy of the DTBP-based AD in a single-filter system for the efficient separation of DNA and proteins within 40 min. This DTBP-based AD syringe filter system showed good rapidity, efficiency, and affordability in the separation of biomolecules from single samples for the early diagnosis and clinical analysis of cancers.
Highlights
Downstream analyses can proceed with improved rapidity, efficiency, sensitivity, and specificity across a wide range of biological applications[24,25]
We demonstrated that this DTBP-based APDMS-functionalized DE (AD) syringe filter system could isolate DNA as well as proteins from various cancer cell lines
We developed the DTBP-functionalized AD through a syringe filter with a PTFE system for DNA and protein separation from a single liquid sample
Summary
Downstream analyses can proceed with improved rapidity, efficiency, sensitivity, and specificity across a wide range of biological applications[24,25]. Syringe filters allow large volumes of samples to be analyzed without the use of complex devices, such as large centrifuges or vacuums. We explored efficient processes for biomolecule (protein and nucleic acid) bioseparation from a single specimen using a functionalized DE and syringe filter with a PTFE membrane. We investigated the use of a DTBP-based APDMS-functionalized DE (AD) syringe filter system for the efficient and inexpensive separation of DNA and proteins, thereby streamlining multiplex biomolecule separation. We applied the DTBP-based AD syringe filter system to isolate DNA and proteins from hepatic, prostatic, breast, and colorectal cancer cell lines, as well as a myelogenous leukemia cell line. We demonstrated the application of the DTBP-based AD syringe filter system for isolating biomolecules from a single specimen, thereby providing a convenient approach for the rapid and affordable bioseparation of clinically applicable biomolecules from scarce samples
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