Abstract
NNew data on germ growth processes of dill seeds formed at the first and second branching orders after exposure to short-term heat stress (40 °C) are presented. Morphometric method and analysis of dill seed embryo growth in dynamics were used. The studies were carried out in 20152017 at the All-Russian Research Institute of Vegetable Growing, a branch of FSBSI FSCVG. Seeds of dill (Anethum graveolens L.) of the variety Centaurus from the first and second branching orders were the object of research. First, seeds were germinated in a thermostat. Then, swollen roots were exposed to a temperature of 40 °C according to the experiment scheme: 0 (control); 1; 2; 3; 4 and 5 days. After incubation, the seeds were transferred to standard conditions (temperature 20 °C) and germinated in Petri dishes on filter paper without light for 21 days. Germ size was measured using image analysis software. Critical embryo length and degree of underdevelopment were determined, and the ratio of embryo length to endosperm length (I Z/E) was calculated. Logistic regression with four parameters: b, c, d, e., was used to construct a germ growth curve. The relationship between the parameters was assessed using Pearson correlation analysis. The differences were considered statistically significant at P≤0.05. The duration of temperature action, which has an inhibitory effect on embryo growth, germination rate, the number of germinated seeds, were revealed. It was found that the embryos of seeds obtained from different branching orders have different sizes and have different intensity of growth under stress and standard conditions. It was shown that the effect of brief temperature (40 °C) on embryo growth depends on branching order and that embryos of seeds of the second branching order are more sensitive to high temperature. Morphometric parameters of the source were shown to play a critical role in the ability of dill seeds to resist the effect of temperature stressor during germination.
Highlights
Исследования проводили в 2015-2017 гг. во Всероссийском научно-исследовательском институте овощеводства – филиале ФГБНУ ФНЦО
Выращенных в открытом грунте по схеме 45×10 см
Pereira R.S., Nascimento W.M., Vieira J.V. Carrot seed germination and vigor in response to temperature and umbel orders // Scientia Agricola
Summary
Исследования проводили в 2015-2017 гг. во Всероссийском научно-исследовательском институте овощеводства – филиале ФГБНУ ФНЦО. Уборку проводили на 50-е сутки после начала цветения зонтиков первого порядка. После инкубации семена переносили в стандартные (температура 20°С) условия и проращивали в чашках Петри на фильтровальной бумаге без доступа света в течение 21 суток. Для исследования динамики роста зародыша семена раскладывали в чашки Петри на фильтровальную бумагу в четырех повторностях по 10 штук для каждых из 21 суток наблюдений. Размер зародыша измеряли с использованием программного обеспечения для анализа изображений Scope Photo Для построения кривой роста зародыша использовали лог-логистическую регрессию с четырьмя параметрами: b – наклон кривой роста зародыша; c – нижняя точка кривой роста зародыша (соответствует начальной длине зародыша); d – верхняя точка кривой роста зародыша (соответствует длине зародыша при максимальном прорастании); e – время (сутки), затраченное на 50%-й прирост от максимальной длины зародыша.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Bulletin of NSAU (Novosibirsk State Agrarian University)
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
