Dikaryotic cell cycle in the phytopathogenic fungus Ustilago maydis is controlled by the DNA damage response cascade

Abstract

In a large group of fungi, mating results in a dikaryon, a cell in which the two nuclei -one from each parent cell- share a single cytoplasm for a period of time without undergoing nuclear fusion. The dikaryon stage is typical in the life cycles of many fungal species primarily in the Basidiomycota, a large group that includes mushrooms, bracket fungi and many phytopathogenic fungi, such as the corn pathogen Ustilago maydis. Recently, we described that in U. maydis two conserved DNA-damage checkpoint kinases, Chk1 and Atr1, work together to control the dikaryon formation. However, how this pathway is activated during the dikaryon formation and how its activation/deactivation is coordinated with the different cell cycle phases is unknown. Here we propose and discuss several hypothesis to address these questions.