Dihydromyricetin attenuates high glucose‐induced oxidative stress in human umbilical vascular endothelial cells (1146.2)

Abstract

Objective To investigate the effects of Dihydromyricetin on high glucose‐induced cell damage in human umbilical vascular endothelial cells (HUVECs).Methods HUVECs were cultured in 5.56 mmol/L glucose as normal control, 5.56mmol/L glucose plus 27.8mmol/L mannitol as an osmotic control, or 33.36 mmol/L high glucose for 48 hours in the absence and presence of Dihydromyricetin, cell viability and apoptosis were detected by CCK‐8 assays and annexin V‐FITC/PI double staining using flow cytometry, respectively. In addition, the intracellular reactive oxygen species (ROS), malondialdehyde (MDA) content and the activity of superoxide dismutase (SOD) and catalase (CAT) in the culture supernatants were measured.Results High concentration of glucose significantly decreased cell viability and the activity of SOD and CAT, while increased the apoptotic rate, the intracellular ROS and the MDA content in HUVECs (P<0.05). Dihydromyricetin pretreatment significantly inhibited the decrease in cell viability and the increase in cell apoptosis induced by high glucose. The ROS and MDA levels were reduced while the SOD and CAT levels were increased after pretreatment with Dihydromyricetin following high glucose stimulation.Conclusion Dihydromyricetin attenuated high glucose‐induced oxidative stress in HUVECs.Grant Funding Source: Supported by The National Natural Science Foundation of China (81102129)