Abstract
Flurorescence anisotropy ( r) was determined in hypoxic/reoxygenating rat heart mitochondria by means of 1,6-diphenyl- 1 ,3,5-hexatriene. During hypoxia, values for r increased, indicating a rigidifled membrane. Membrane fluidity was increased when 20 pmol to 2 nmol/mg protein of dihydrolipoic acid (DHL) was present during hypoxia. Dithiothreitol (DTT) or 2-mercaptopropionyl-glycin (MPG) at similar concentration did not result in membrane fluidization under these conditions. Peroxidative damage was induced in mitochondria by H 2O 2. In the presence of 2 nmol/mg protein of DHL, malondialdehyde production was decreased by 50%. DTT or MPG brought about only a 25% decrease. These results were corroborated by spin label studies with 4-maleimido-TEMPO and 5-proxylnonane (5-P-9); H 2O 2 induced a decrease in fluidity in the region of labeled thiol groups and an increase in polarity sensed by 5-P-9. DHL proved efficient in reducing such fluidity and polarity changes.
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