Abstract
The effect of methotrexate on dihydrofolate reductase activity of normal and leukemic human leukocytes has been studied with folate as enzyme substrate. In a delayed effect, methotrexate increased dihydrofolate reductase activity in normal leukocytes and in chronic myelogenous leukemia. Enzyme activity was elevated in two of seven patients with chronic lymphocytic leukemia, but not in patients with acute leukemia. The administration of methotrexate inhibits the “drug-induced” elevation of enzyme activity in normal leukocytes and in chronic myelogenous leukemia. The incorporation of deoxyuridine and thymidine into DNA was inhibited by methotrexate in childhood leukemia. In adults with leukemia, incorporation of deoxyuridine but not thymidine was inhibited. This difference beween adults and children, which must reflect a second effect of methotrexate, may occur as a result of the inhibition of purine synthesis in children.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
