Abstract
A crystalline glycosidic substance was isolated, using enzyme-inhibiting techniques, from Digitalis lanata that was grown from seeds obtained from A. Stoll. This glycosidic material had physical and chemical properties that very closely resembled those of a glycosidic material obtained from Digitalis lanata that had been propagated over a number of years in the botanical gardens of the University of Minnesota. This glycosidic material was previously characterized as being composed chiefly of acetyl digoxin. When fresh leaves were incubated for eight, twenty-four, and forty-eight hours, either the same crystalline glycosidic material was obtained as previously described or no crystalline material could be isolated. Digitalis lanata leaves that had been improperly stored for seven months failed to yield any crystalline glycosidic material. By using Stoll's enzyme-inhibiting techniques, a crystalline glycosidic material was obtained that appeared to be identical to that obtained by the new techniques described. In addition, the Stoll technique was more tedious and difficult.
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