Abstract
Canine coronavirus (CCoV) is an important pathogen that affect dogs. Here we assessed a digital real-timebased PCR (dPCR) for the detection of CCoV from infected AF-72 cells and directly from faeces from 100 symptomatic dogs.
Highlights
Canine coronavirus (CCoV) and coronaviruses of cats and pigs are closely and compose a unique viral species [1,2,3]
CCoV-II has been subdivided into CCoV-IIa and CCoV-IIb with no association with clinical disease in dog [4,5]
In order to characterize CCoV genotypes detected after virus isolation sequencing of S gene was performed as described previously [7,14]
Summary
Canine coronavirus (CCoV) and coronaviruses of cats and pigs are closely and compose a unique viral species [1,2,3]. A highly virulent strain (pantropic CCoV-IIa) was isolated during an outbreak of fatal, systemic disease in puppies in Italy [6]. Upon the emergence of pantropic CCoV-IIa strains, monitoring of these novel coronaviruses in dog populations has become more important [7,8,9,10]. The diagnostic of CCoV, due to difficulty of virus isolation and electron microscopy of viral particles from clinical samples, is based on virus molecular detection [11,12]. First description of quantitative real-time TaqMan® RT-PCR (reverse transcriptasepolymerase chain reaction) to detect and quantify RNA CCoV revealed high sensitivity and simplicity [13]. DPCR, a newer technology than qPCR, provides absolute quantification without need for standard curves as well as high sensitivity, reproducibility and semi-automation [12]
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