Digital image analysis to measure lesion area of cucumber anthracnose by Colletotrichum orbiculare

Abstract

A method was developed using scanned computer images and software programs to measure the lesion area on leaves with cucumber anthracnose caused by Colletotrichum orbiculare. After cucumber plants were inoculated with various concentrations of conidia, lesions on diseased leaves were scanned, manipulated with Adobe Photoshop 6.0, and measured using the blob analysis feature in Matrox Inspector 2.2. The method requires relatively low-cost equipment including a scanner, a personal computer, and two programs: Adobe Photoshop 6.0 and Matrox Inspector 2.2. Because stored images are used, the lesion area can be measured as time permits. Processing the images requires about 3 min per sample. The image assessment accurately detected anthracnose lesions on cucumber leaves and could be applied to other foliar necrotic or spot diseases.