Abstract
Digital holography is a well-accepted method for phase imaging. However, the phase of the object is always embedded in aberrations. Here, we present a digital holographic phase imaging with the aberrations fully compensated, including the high order aberrations. Instead of using pre-defined aberration models or 2D fitting, we used the simpler and more flexible 1D fitting. Although it is 1D fitting, data across the whole plane could be used. Theoretically, all types of aberrations can be compensated with this method. Experimental results show that the aberrations have been fully compensated and the pure object phase can be obtained for further studies.
Highlights
Digital holographic phase imaging (DHPI) is widely used to quantitatively detect phase information with axial sensitivity in nanoscale [1,2]
If standard deviation (STD) is larger than a threshold value t, we find the direction u along which the STD is maximum using grid search
A polarizing beam splitter (PBS) divided the light into two beams, one reflected by a spatial light modulator (SLM, Holoeye, Pluto-VIS) worked as the reference wave, while the other one reflected by the sample worked as the object wave
Summary
Digital holographic phase imaging (DHPI) is widely used to quantitatively detect phase information with axial sensitivity in nanoscale [1,2] With this technique, transparent samples such as cells can be visualized [1,3], without contrast agents (stains or fluorescence dyes) and in a completely noninvasive way. One solution is two-dimensional (2D) surface fitting using pre-defined (high order aberrations included) aberration models [18,19], with the most acceptable technique being the 2D Zernike Polynomials fitting (ZPF) [18] Many of these techniques, perform 2D fitting across the entire field of view (FOV) without considering the presence of object phase [18,19], leading to somewhat suboptimal results. Experimental results show that the phase aberrations can be fully compensated and the pure phase of object can be obtained for further analysis
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