Abstract
Most biological micro-objects are phase objects that do not change the intensity of the radiation transmitted through them and are inaccessible to direct observation. Such methods of visualizing phase micro-objects are Zernike’s phase contrast method and the interference contrast method. However the problem of 3D visualization of phase microobjects has not been solved in classical microscopy. Electron microscopy makes it possible to obtain an image of a phase micro-object with high resolution. But this method requires special sample preparation and does not make it possible to study untreated micro-objects. The appearance of holography opened up new possibilities in the microscopy of phase micro-objects. The problem of the 3D visualization of phase micro-objects was solved by combining holographic methods with the methods of computerised image processing [1].
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