Digital droplet LAMP as a microfluidic app on standard laboratory devices

Friedrich Schuler,Sebastian Hin,Nils Paust,Simon Wadle,Felix Von Stetten,Clara Siber,Roland Zengerle

doi:10.1039/c6ay00600k

Friedrich Schuler, Sebastian Hin + Show 5 more

Open Access

https://doi.org/10.1039/c6ay00600k

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Abstract

Digital droplet LAMP is performed on a disposable chip (DropChip) with the size of a microscope slide using only standard laboratory devices.

Highlights

Digital ampli cation of nucleic acids allows for absolute quanti cation of nucleic acids with or without the need for standards and the possibility to detect single molecules
Following the micro uidic app concept,[24,25] we present a micro uidic disposable cartridge (DropChip) in the standardized format of a microscope slide (ISO 8037-1), which allows for digital droplet LAMP using only commercially available laboratory devices
The in uence of DNA denaturation was tested by performing a droplet loopmediated isothermal amplification (ddLAMP) experiment with two aliquots of target DNA from the same vial

Summary

Introduction

Digital ampli cation of nucleic acids allows for absolute quanti cation of nucleic acids with or without the need for standards and the possibility to detect single molecules. The use of isothermal ampli cation techniques for digital ampli cation has been introduced. They o en enable shorter reaction times than the dPCR. They typically use less electrical power than the dPCR, as they run at lower temperatures and require no temperature changes. This can reduce the price of the needed devices and can be advantageous when battery powered, portable devices are needed, e.g. for resource-poor settings.

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