Abstract

In this paper, a digital bio-dosimeter (DBD) with a square window size of 7 × 7 pixels is proposed for the quantitative detection of the deformed biological cells irradiated by X-rays with different energies and γ-rays from 60Co. The basic idea of the DBD is to compute the energy of the bio-dosimeter window size by summing up the entire gray levels. Peripheral blood films (PBFs) from male albino rats irradiated by ionizing radiations are prepared and photographed using an Automatic Image Contour Analysis system with high magnification to produce a 2D image. The surf function in MATLAB is used to convert the 2D image to a 3D shaded surface to resample the date more finely. The proposed DBD shows that the computed energies on the boundaries of the normal RBCs are nearly the same, while the computed energies on the boundaries of the deformed cells are significantly larger.

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