Abstract

An experiment was carried out to evaluate the requirement of digestible methionine for growing pullets at growth phase (7 to 12 weeks of age). A completely randomized design was distributed in five treatments, six replicates, and 15 pullets per experimental unit. 450 Dekalb White pullets from the 7th weeks of age, with an average initial weight of 313.14 ± 12.49 g were used. Dietary treatments consisted in five diets supplemented with DL-Methionine which resulted in five levels of digestible methionine (0.266, 0.294, 0.322, 0.350, and 0.378 %). Performance, serological blood, histopathology and histomorphometry data were evaluated. Quadratic responses were observed for final live weight (p < 0.0143), weight gain (p < 0.0073), feed conversion ratio (p < 0.0058), glycogen deposition in the liver (p < 0.0001), gamma-glutamyl transferase enzyme activity (p < 0.0008), and villus height (p < 0.0024) with digestible dMet levels. In conclusion, the use of 0.343 % dMet, corresponding to a dMet:dLys ratio 55, is recommended for white-egg pullets from 7 to 12 weeks of age.

Highlights

  • Dietary treatments consisted in five diets supplemented with DL-Methionine which resulted in five levels of digestible methionine (0.266, 0.294, 0.322, 0.350, and 0.378 %)

  • Methionine is amino acid involved in the control of some functions in the bird’s body that potentiate weight gain and growth rates (Del Vesco et al, 2013), protection against liver damage (Wu et al, 2012), intestinal development and morphology (Shen, Ferket, Park, Malheiros & Kim, 2015), and formation and functioning of the immune system (Jankowski, Kubinska & Zdunczyk, 2014)

  • Based on the results obtained in this study, it can be inferred that the best level found for feed conversion is related to the better use of the dietary nutrients for weight gain

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Summary

Introduction

Methionine is the first limiting amino acid for laying hens, and can be effective for the protein synthesis, for the development of muscles, organs, feather, beaks, and for the production and quality of eggs.The update of dietary requirements for layer pullets was justified by improvement the lineages, with superior productive characteristics as well as unprecedented knowledge of the nutrition, management, and ambience fields (D’Agostini et al, 2012).Methionine is amino acid involved in the control of some functions in the bird’s body that potentiate weight gain and growth rates (Del Vesco et al, 2013), protection against liver damage (Wu et al, 2012), intestinal development and morphology (Shen, Ferket, Park, Malheiros & Kim, 2015), and formation and functioning of the immune system (Jankowski, Kubinska & Zdunczyk, 2014).The proper methionine nutritional level boosts productive performance rates, tissue formation, and the functioning of physiological systems, and maximizes the preparation of pullets for the subsequent developer and egg-laying phases.the Granja Planalto (2009) manual and Rostagno et al (2017) recommended dMet levels of 0.52g 100g-1 for white-egg pullets from 6 to 10 weeks of age, and 0.44g 100g-1 digestible methionine for white-egg pullets from 5 to 10 weeks of age, respectively.This study aimed to determine the digestible methionine requirement for white-egg pullets from 7 to 12 weeks of age. Methionine is the first limiting amino acid for laying hens, and can be effective for the protein synthesis, for the development of muscles, organs, feather, beaks, and for the production and quality of eggs. Methionine is amino acid involved in the control of some functions in the bird’s body that potentiate weight gain and growth rates (Del Vesco et al, 2013), protection against liver damage (Wu et al, 2012), intestinal development and morphology (Shen, Ferket, Park, Malheiros & Kim, 2015), and formation and functioning of the immune system (Jankowski, Kubinska & Zdunczyk, 2014). The proper methionine nutritional level boosts productive performance rates, tissue formation, and the functioning of physiological systems, and maximizes the preparation of pullets for the subsequent developer and egg-laying phases.

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