Diffusion time dependence, power-law scaling, and exchange in gray matter

Abstract

Characterizing neural tissue microstructure is a critical goal for future neuroimaging. Diffusion MRI (dMRI) provides contrasts that reflect diffusing spins’ interactions with myriad microstructural features of biological systems. However, the specificity of dMRI remains limited due to the ambiguity of its signals vis-à-vis the underlying microstructure. To improve specificity, biophysical models of white matter (WM) typically express dMRI signals according to the Standard Model (SM) and have more recently in gray matter (GM) taken spherical compartments into account (the SANDI model) in attempts to represent cell soma. The validity of the assumptions underlying these models, however, remains largely undetermined, especially in GM. To validate these assumptions experimentally, observing their unique, functional properties, such as the b−1/2 power-law associated with one-dimensional diffusion, has emerged as a fruitful strategy. The absence of this signature in GM, in turn, has been explained by neurite water exchange, non-linear morphology, and/or by obscuring soma signal contributions. Here, we present diffusion simulations in realistic neurons demonstrating that curvature and branching does not destroy the stick power-law behavior in impermeable neurites, but also that their signal is drowned by the soma signal under typical experimental conditions. Nevertheless, by studying the GM dMRI signal's behavior as a function of diffusion weighting as well as time, we identify an attainable experimental regime in which the neurite signal dominates. Furthermore, we find that exchange-driven time dependence produces a signal behavior opposite to that which would be expected from restricted diffusion, thereby providing a functional signature that disambiguates the two effects. We present data from dMRI experiments in ex vivo rat brain at ultrahigh field of 16.4T and observe a time dependence that is consistent with substantial exchange but also with a GM stick power-law. The first finding suggests significant water exchange between neurites and the extracellular space while the second suggests a small sub-population of impermeable neurites. To quantify these observations, we harness the Kärger exchange model and incorporate the corresponding signal time dependence in the SM and SANDI models.