Abstract
The dynamics of lipids and proteins are crucial in the mechanisms of cellular functions. The robustness of fluorescence correlation spectroscopy (FCS) makes it possible to quantify the diffusion and molecular interactions at nanomolar concentration in biological systems. We developed novel, economical methods with the use of super-continuum laser and spectral deconvolution with experiment-specific excitation wavelengths and emission spectra for revealing the interactions between up to four spectrally overlapping fluorophores.
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