Abstract
Purpose. Diffusion coefficients of various retinoids have not been measured previously. It is important to know the diffusion coefficients of the retinoids because this property might be rate-limiting in dark adaptation. Also, retinoid diffusion is important to explore given that rhodopsin regeneration is not impaired in IRBP knockout mice. Methods. Measurements of lateral diffusion coefficients (D) of 9-cis-retinal, all-trans-retinal, and all-trans-retinol were made by Fourier transform pulsed-gradient spin-echo NMR measurements (FT-PGSE NMR) in several solvents. Also, 3 H-all-trans-retinoic acid was used to measure diffusion from an aqueous agarose matrix and absorption into a toluene based scintillation fluid in a biphase assay. Results. In a 1:1 mixture of CD 3 OD:D 2 O the D’s of the retinoids were, 2.4 to 3.0 × 10 -6 cm 2 /s. In the biphase assay, 3 H-all trans-retinoic acid exhibited a diffusion coefficient of 2.3 × 10 -6 cm 2 /s. Conclusions. The lower than expected D for retinoids and our calculations suggest that mechanisms in addition to pure aqueous diffusion may be needed to account for normal rhodopsin regeneration rates in the mammalian retina.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
