Abstract
Reduction in scattering, high absorption, and spectral features of tissue constituents above 1000 nm could help in gaining higher spatial resolution, penetration depth, and specificity for in vivo studies, opening possibilities of near-infrared diffuse optics in tissue diagnosis. We present the characterization of collagen absorption over a broadband range (500 to 1700 nm) and compare it with spectra presented in the literature. Measurements were performed using a time-domain diffuse optical technique. The spectrum was extracted by carefully accounting for various spectral distortion effects, due to sample and system properties. The contribution of several tissue constituents (water, lipid, collagen, oxy, and deoxy-hemoglobin) to the absorption properties of a collagen-rich in vivo bone location, such as radius distal in the 500- to 1700-nm wavelength region, is also discussed, suggesting bone diagnostics as a potential area of interest.
Highlights
In recent years, diffuse optical spectroscopy (DOS) is advancing as a promising tool for noninvasive quantification of tissue constituents in pathological diagnostics.[1,2,3,4] Traditionally, in vivo diffuse optical measurements have been performed, at most, in the 600- to 1100-nm window.[5,6,7] This is due to high hemoglobin absorption below 600 nm and dominant water absorption combined with lack of suitable detection technology above 1100 nm
Recent investigations emphasize the advantage of high penetration depth and enhanced constituent specificity in the short wave infrared (SWIR) 1100- to 1700-nm range.[8,9,10]
The absorption and reduced scattering spectra of collagen measured by our system are shown in Fig. 2
Summary
Diffuse optical spectroscopy (DOS) is advancing as a promising tool for noninvasive quantification of tissue constituents in pathological diagnostics.[1,2,3,4] Traditionally, in vivo diffuse optical measurements have been performed, at most, in the 600- to 1100-nm window.[5,6,7] This is due to high hemoglobin absorption below 600 nm and dominant water absorption combined with lack of suitable detection technology above 1100 nm. Recent investigations emphasize the advantage of high penetration depth and enhanced constituent specificity in the short wave infrared (SWIR) 1100- to 1700-nm range.[8,9,10] Importantly, the lack of systematic characterization of tissue constituents in the SWIR range prevents its widespread use for in vivo applications
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