Abstract
We present a novel optical device developed for the monitoring of dynamic behavior in extended 3D-tissue models in various culture environments based on variations in their speckle patterns. The results presented point out the benefit of the technology in terms of detection, accuracy, sensitivity and a reasonable read-out speed as well as reproducibility for the measurements and monitoring of cardiac contractions. We show that the optical read-out technology is suitable for long time monitoring and for drug screening. The method is discussed and compared to other techniques, in particular calcium imaging. The device is flexible and easily adaptable to 2D and 3D-tissue model screenings using different culture environments. The technology can be parallelized for automated read-out of different multi-well-plate formats.
Highlights
Various important fields of work in modern pharmacy and biotechnology use unbiased mass tests of compounds on biological model systems in order to find candidates with favored effects
We present a novel optical device developed for the monitoring of dynamic behavior in extended 3D-tissue models in various culture environments based on variations in their speckle patterns
We presented a novel optical device developed for the monitoring of dynamic behavior in 3D-tissue models based on variations in their Speckle patterns
Summary
Various important fields of work in modern pharmacy and biotechnology use unbiased mass tests of compounds on biological model systems in order to find candidates with favored effects. Since most biological processes are not confined to single cells, but have considerable interplay to higher levels of organization, such as tissue, organs, organisms and even populations, cultures of isolated cells are vastly oversimplified models in the majority of cases. This will result in poor correlation of screening results with real-life scenarios [3]. For this reason a paradigm shift is going on in biomedical screening towards the application of higher organized, three dimensional tissue models that reflect native tissue architecture and functionality. A prominent cultivation technique to receive homogeneously sized single- or multicellular aggregates that match physiological appearances is the hanging drop, where cells aggregate in small volumes to 3D constructs due to gravity and the absence of solid interfaces [6,7]
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