Abstract
Friend leukemia cells differentiate when any one of a variety of cryoprotective agents are added to their suspension culture media. While dimethyl sulfoxide (DMSO), dimethylacetamide (DMA) and tetramethylurea (TMU) induce a comparable degree of erythroid maturation their effects on the acid soluble ribonucleotide pools differ. DMSO induced differentiation is accompanied by a 70–80% reduction in pool size while DMA and TMA cause only a 15–20% reduction. Furthermore, inhibition of DMSO-induced differentiation by BUdR does not prevent the DMSO-induced reduction in pool size. These data suggest that the decline in pool size is a toxic effect unrelated to differentiation and these effects should be considered when the biological effects of DMSO-induced differentiation are considered.
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