Abstract
Duck Tembusu virus (DTMUV), the causative agent of egg-drop syndrome, has caused substantial economic losses to duck industry. DTMUV infection leads to profound changes of host cells, including transcriptome and proteome. However, the lncRNA expression profile and the biological function of lncRNA have not been revealed. Therefore, DTMUV was used to inoculate duck embryo fibroblast cells (DEFs) for high-throughput RNA-sequencing (RNA-Seq). The results showed that 34 and 339 differently expressed lncRNAs were, respectively, identified at 12 and 24 h post-infection (hpi). To analyze their biological functions, target genes in cis were searched and the regulatory network was formed. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the target genes were strongly associated with immune system, signaling molecular and interaction, endocrine system, and signal transduction. The differently expressed lncRNAs were selected and verified by quantitative real-time polymerase chain reaction (RT-qPCR). Our study, for the first time, analyzed a comprehensive lncRNA expression profile in DEFs following DTMUV infection. The analysis provided a view on the important roles of lncRNAs in gene regulation and DTMUV infection.
Highlights
In 2010, a novel disease characterized by a significant decline in egg-drop production broke out in many duck farms across Chain [1]
duck embryo fibroblast cells (DEFs) were infected with Duck Tembusu virus (DTMUV) at a multiplicity of infection (MOI) of 3
The DEFs at 12 and 24 hpi were harvested for RNA-Seq
Summary
In 2010, a novel disease characterized by a significant decline in egg-drop production broke out in many duck farms across Chain [1]. The disease, diagnosed as duck hemorrhagic ovaritis, was proven to be caused by DTMUV [2,3,4]. DTMUV, similar to other flaviviruses, is a single-stranded, positive-sense RNA virus with an approximately 11 kb genome. It can infect ducks and geese [5], chickens [6], sparrows [5], pigeons [7], and mice [6]. A wide spectrum of mammalian cells, including A549, BHK21, Hela, Vero, and SH-SY5Y, exhibit obvious cytopathic effects (CPEs) after DTMUV infection [8]. Our previous study showed that CPEs were appeared on HEK293 when the cells were infected with DTMUV, which implies that the virus with the possibility to infect human can potentially threaten human health [9]
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