Abstract
Objective To study the differentiation types of microglia induced by macrophage colony-stimulating factor(M-CSF). Methods Rat microglia cultured in vitro were inoculated on 6-well plates and divided into 3 groups(n=4 each)using a random number table method when cell confluence reached 70%: blank control group(C group), vehicle control group(P group)and M-CSF group.Group P was incubated with phosphate buffer solution for 7 days and group M-CSF with 20 ng/ml M-CSF for 7 days.The expression of a specific M1 phenotype marker tumor necrosis factor-alpha(TNF-α)and specific M2 phenotype markers interleukin-10(IL-10)and brain-derived neurotrophic factor(BDNF)was determined by Western blot. Results Compared with C group, the expression of IL-10 and BDNF was significantly up-regulated(P 0.05), and no significant change was found in the expression of TNF-α, IL-10 or BDNF in P group(P>0.05). Conclusion M-CSF can induce microglia to differentiate into a M2 phenotype. Key words: Macrophage colony-stimulating factor; Microglia; Cell differentiation
Published Version
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