Abstract
A method was found by means of which two types of granular cells in the pars nervosa of the hypophysis cerebri could be preserved in permanent preparations so as to retain the appearance these cells presented in fresh tissue and in tissue cultures. The essential points of the technic were the fixation for 24 hours of the hypophysis in a solution composed of 2 parts of 3% potassium bichromate plus one part of a one-half saturated solution of bichloride of mercury in 95% alcohol. Sections of this material were prepared using dioxan in place of the higher alcohols and xylene. The sections were stained by means of Mallory's connective tissue stain leaving the sections in the fuchsin solution for 30 minutes and in the mixture of aniline blue, orange G and phosphotungstic acid for 1 to 24 hours.
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