Abstract

The mechanism of somatic and germinal nuclear differentiation after conjugation was investigated in Paramecium caudatum by the method of microsurgery. The results show that the stage of the determination for this differentiation is immediately after the third (last) nuclear division of the fertilization nucleus and that the differentiation seems to be closely associated with a brief localization of the nuclei near the opposite ends of the cell, with somatic nuclei (macronuclei) in the posterior region and the germinal nucleus (micronucleus) in the anterior region. The different fates of the postzygotic nuclei may depend on their different positions, which are a consequence of anteroposterior differentiation of the cytoplasm. The elimination of a prezygotic somatic nucleus suggests the possibility of a control by the old somatic nucleus over the differentiation of the new somatic nucleus.

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