Differentiation of odontoblasts in mouse dental papillae recombined with normal or chemically-treated dentinal matrices

Abstract

Separation and recombination experiments were made with manually or trypsin-dissociated dental papillae (day 15, 16, 17, 18 in utero and 2, 7, 14 postnatal) and manually isolated hard tissues of the third molar crown (14 postnatal days). Several series of hard tissues were further treated with citric acid, hyaluronidase or sodium hypochlorite. The recombinations were transplanted into the subcutaneous tissue of new-born mice. Grafts were removed 7, 14 and 21 days later and prepared for light and electron microscopy. Whatever the age of the papilla and whatever the treatment of the crowns, well-characterized odontoblasts differentiated and deposited new layers of tubular dentine, except when the recombined dental papilla was 15 days old. These findings indicate that odontoblasts are very early committed (since day 16 in utero) and that they may differentiate in dental papillae in contact with chemically altered dentinal matrices.