Differentiation of muscle fiber types in the teleost Brachydanio rerio, the zebrafish. Posthatching development.

Abstract

The trunk musculature of adult zebrafishes contains three major fiber types: adult red, intermediate, and white; and two minor populations: red muscle rim and scattered intermediate fibers. In this paper, the post hatching development of these muscle fiber types was studied by means of immunohistochemistry, using anti-myosin sera. Just hatched larvae contain two muscle fiber populations: embryonic red and white, which give rise to the red muscle rim and the intermediate fibers respectively. Adult red fibers arise post hatching as a new separate population with distinct myosin properties. The differentiation of these fiber types occurs within the first four weeks after fertilization, when the adult pattern of peripheral axon bundles has become established. Differences in the muscle fiber type composition between the midbody and the tail myotomes become apparent in two month old fries. The number of scattered intermediate fibers increases from rostral to caudal, the opposite holds for the red muscle rim fibers. The red and intermediate area is triangular in the midbody; in the tail part it is stretched out along the lateral surface of the myotomes. These changes are considered as adaptations to improve the efficiency of the swimming performance.