Abstract
Menstrual blood stem cells (MensSCs) have enormous potential as a source for cell replacement therapies. Since there is a major concern in utilization of nanofibers in tissue engineering of stem cells, we examined the potential of MensSCs to differentiate into hepatocytes, using different protocols and compare cells, with two-dimensional (2D) and three-dimensional (3D) culture systems. Cell characterization experiments of MensSCs have demonstrated that they are multipotent stem cells similar to mesenchymal stem cells, which can successfully differentiate into osteogenic and adipogenic lineages. The efficiency of the cells on the scaffold was appraised by scanning electron microscopy (SEM), MTT assay, and hematoxylin and eosin (H&E) staining. Thereafter, the differentiation protocols were developed by hepatocyte growth factor (HGF) and oncostatin M (OSM) with serum-supplemented or serum-free culture media up to 30 days. Immunofluorescence analysis and ELISA assay revealed the expression of albumin (ALB) in differentiated cells. Hepatocyte-like cells expressed liver-specific gene such as albumin(ALB), α-fetoprotein (AFP), tyrosine aminotransferase (TAT) and cytochrome P450 subunit 7a1 (Cyp7a1) at mRNA levels. In conclusion, the evidences presented in this study show that the nanofiber scaffold and MensSCs may provide a source of differentiated cells for treatment of liver diseases.
Highlights
Hepatocyte transplantation could be a superseded solution for people on transplant waiting lists as a replacement for whole organ transplantation [1]
The correlation function of the menstrual cycle with Oct-4 as a self-renewal marker of undifferentiated embryonic stem was shown [8]. This cell compared with embryonic stem cells, the markers expressed in the endometrium do not fully exhibit the embryonic markers leading to the formation of teratoma [9] [10]
Dulbecco’s Modified Eagle’s Medium (DMEM, pH 7.4), fetal bovine serum (FBS), 2 mM l-glutamine, penicillin and streptomycin, non-essential amino acids, fangizone, 0.05% trypsin-EDTA were obtained from Gibco Life Technologies Co.PCL (70,000 - 90,000 KDa), 3-(4,5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), insulin-transferrin-selenium + 1 (ITS + 1) premix, Epithelial growth factor (EGF), basic fibroblast growth factor, nicotine amid (NTA), Hepatocyte growth factor (HGF), dexamethasone (Dex) were purchased from Sigma-Aldrich Co. and Ficoll-Paque was provided from Amersham Bioscience Co. (Sweden)
Summary
Hepatocyte transplantation could be a superseded solution for people on transplant waiting lists as a replacement for whole organ transplantation [1]. Stem cells derived from menstrual blood have noninvasive methods and no ethical problems of embryonic stem cells. These cells illustrate stem cell-like phenotypic markers and performance high potential for self-renewal, plasticity. The correlation function of the menstrual cycle with Oct-4 as a self-renewal marker of undifferentiated embryonic stem was shown [8] This cell compared with embryonic stem cells, the markers expressed in the endometrium do not fully exhibit the embryonic markers leading to the formation of teratoma [9] [10]. A lot of studies have introduced this population as pluripotent cells They are capable of differentiating into specific-tissue cells of three germ layers both in vitro and in vivo, and play an important role in improving the disease status of study models [11]. In this study differentiation potential of MensSCs to hepatocyte-like cell on nanofiber scaffold was experimented
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