Differentiation of human induced pluripotent stem cells to authentic macrophages using a defined, serum-free, open-source medium.

Abstract

Main text(Stem Cell Reports 16, 1735–1748; 2021)The authors would like to make two corrections to the supplemental information for this manuscript. First, the formulation of XVIVO medium is stated to be the same as previously published by van Wilgenburg et al. (2013), and OXM is subsequently based on this formulation. In Allshire and Madhani, 2018van Wilgenburg B. Browne C. Vowles J. Cowley S.A. Efficient, long term production of monocyte-derived macrophages from human pluripotent stem cells under partly-defined and fully-defined conditions.Nat. PLoS One. 2013; 8 (e71098)Google Scholar the concentration of M-CSF used was 100 ng/mL; however, in the originally published version of our supplemental information, 50 ng/mL M-CSF was reportedly used. This value was incorrect as written, and the concentration was in fact 100 ng/mL, the same as in Wilgenburg et al. (2013). Therefore, Table S2 in the supplemental information has been corrected to reflect this, stating a final concentration of 100 ng/mL M-CSF in both OXM and XVIVO media. Secondly, in the originally published version of this paper, OXM Macrophage medium is described to be the same as OXM differentiation medium with only Tropolone and IL-3 omitted. However, in the originally published version of Table S2 in the supplemental information, the composition of OXM Macrophage medium is listed as 96.5% Advanced DMEM/F-12, 2mM GlutaMAX, 15mM HEPES, 10 ng/mL M-CSF, and 1% Penicillin-Streptomycin. The correct formulation is 96.5% Advanced DMEM/F-12, 2mM GlutaMAX, 15mM HEPES, 5 μg/mL human recombinant Insulin solution, 100 ng/mL M-CSF, and 1% Penicillin-Streptomycin. The supplemental information has now been corrected with the manuscript online and the authors sincerely apologize for any confusion these errors may have caused. Main text(Stem Cell Reports 16, 1735–1748; 2021)The authors would like to make two corrections to the supplemental information for this manuscript. First, the formulation of XVIVO medium is stated to be the same as previously published by van Wilgenburg et al. (2013), and OXM is subsequently based on this formulation. In Allshire and Madhani, 2018van Wilgenburg B. Browne C. Vowles J. Cowley S.A. Efficient, long term production of monocyte-derived macrophages from human pluripotent stem cells under partly-defined and fully-defined conditions.Nat. PLoS One. 2013; 8 (e71098)Google Scholar the concentration of M-CSF used was 100 ng/mL; however, in the originally published version of our supplemental information, 50 ng/mL M-CSF was reportedly used. This value was incorrect as written, and the concentration was in fact 100 ng/mL, the same as in Wilgenburg et al. (2013). Therefore, Table S2 in the supplemental information has been corrected to reflect this, stating a final concentration of 100 ng/mL M-CSF in both OXM and XVIVO media. Secondly, in the originally published version of this paper, OXM Macrophage medium is described to be the same as OXM differentiation medium with only Tropolone and IL-3 omitted. However, in the originally published version of Table S2 in the supplemental information, the composition of OXM Macrophage medium is listed as 96.5% Advanced DMEM/F-12, 2mM GlutaMAX, 15mM HEPES, 10 ng/mL M-CSF, and 1% Penicillin-Streptomycin. The correct formulation is 96.5% Advanced DMEM/F-12, 2mM GlutaMAX, 15mM HEPES, 5 μg/mL human recombinant Insulin solution, 100 ng/mL M-CSF, and 1% Penicillin-Streptomycin. The supplemental information has now been corrected with the manuscript online and the authors sincerely apologize for any confusion these errors may have caused. (Stem Cell Reports 16, 1735–1748; 2021) The authors would like to make two corrections to the supplemental information for this manuscript. First, the formulation of XVIVO medium is stated to be the same as previously published by van Wilgenburg et al. (2013), and OXM is subsequently based on this formulation. In Allshire and Madhani, 2018van Wilgenburg B. Browne C. Vowles J. Cowley S.A. Efficient, long term production of monocyte-derived macrophages from human pluripotent stem cells under partly-defined and fully-defined conditions.Nat. PLoS One. 2013; 8 (e71098)Google Scholar the concentration of M-CSF used was 100 ng/mL; however, in the originally published version of our supplemental information, 50 ng/mL M-CSF was reportedly used. This value was incorrect as written, and the concentration was in fact 100 ng/mL, the same as in Wilgenburg et al. (2013). Therefore, Table S2 in the supplemental information has been corrected to reflect this, stating a final concentration of 100 ng/mL M-CSF in both OXM and XVIVO media. Secondly, in the originally published version of this paper, OXM Macrophage medium is described to be the same as OXM differentiation medium with only Tropolone and IL-3 omitted. However, in the originally published version of Table S2 in the supplemental information, the composition of OXM Macrophage medium is listed as 96.5% Advanced DMEM/F-12, 2mM GlutaMAX, 15mM HEPES, 10 ng/mL M-CSF, and 1% Penicillin-Streptomycin. The correct formulation is 96.5% Advanced DMEM/F-12, 2mM GlutaMAX, 15mM HEPES, 5 μg/mL human recombinant Insulin solution, 100 ng/mL M-CSF, and 1% Penicillin-Streptomycin. The supplemental information has now been corrected with the manuscript online and the authors sincerely apologize for any confusion these errors may have caused. Differentiation of human induced pluripotent stem cells to authentic macrophages using a defined, serum-free, open-source mediumVaughan-Jackson et al.Stem Cell ReportsJune 24, 2021In BriefVaughan-Jackson et al. sought to bring transparency and malleability to the composition of growth media for human iPSC-derived macrophages. They describe a new, simple, defined, and open-sourced medium which supports the growth of macrophages. Building upon previous methodologies for iPSC-derived macrophage differentiation, they improve terminal differentiation and provide fully competent authentic tissue-derived macrophages. Full-Text PDF Open Access