Differentiation of fibre types in an extraocular muscle of the rat

Abstract

Mammalian extraocular muscles possess greater variation in structural and physiological properties than any other muscle. The superior rectus muscle of the adult rat contains five morphological fibre types. The differentiation of the muscle into these fibre types in embryonic and postnatal rats were studied by light and electron microscopy, and the distribution of each developing fibre type with its distinctive features was mapped. The muscle of the 18-day embryos did not display the specific structural fibre types that were observed in the adult muscle. Newborn rat muscle exhibited some differentiation that included scattered small-diameter fibres with large myofibrils (fibre type 'B'). As development proceeded, another small-diameter fibre type with small myofibrils (fibre type 'A') appeared in the 6-day postnatal rat muscle. By the end of the first week of development neuromuscular junctions were in evidence in these two fibre types. Postsynaptic folds were rare in the large-fibril fibre, and folds were extensive in the small-fibril fibre. The medium- (fibre type 'C') and large-diameter (fibre type 'D') fibres were fully differentiated with small myofibrils and abundant sarcoplasmic reticulum (SR) by the second week of the development. SR was most abundant in the large-diameter fibre, which constituted the predominant global fibre type in the adult muscle. The postsynaptic folds in the neuromuscular junctions of these two fibre types were highly developed, although the innervation did not extend widely in the global region of the muscle. The last fibre type (fibre type 'E') was fully differentiated with the largest myofibrils, a small amount of SR, and simple neuromuscular junction by the third week of the postnatal development. The superior rectus muscle of the four-week-old rat was differentiated with all fibre types present in the adult muscle. During the third to sixth, and final, week of development, the other types described above exhibited extensive differentiation of characteristic structural features.