Abstract
Extremely low frequency magnetic fields (ELF MF) have a wide variety of effects in biological systems. Rat chromaffin cells in vitro show morphological and biochemical changes when exposed to ELF MF similar to those produced by nerve growth factor (NGF). To determine whether ELF MF alters catecholamine (CA) release, we used a culture of postnatal rat chromaffin cells which was differentiated by NGF or ELF MF for 7 days. Levels of catecholamine on media culture were detected by high pressure liquid chromatography with electrochemical detection (HPLC-ED) analysis. The results showed that differentiated cells released more dopamine than adrenaline, while chromaffin undifferentiated cells released more adrenaline than dopamine. In both cases noradrenaline release did not change. The results are discussed in terms of the role of Ca 2+ or some enzymes in the changes in messenger ratios.
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