Differentiation of Candida dubliniensis on CHROM agar and Pal's agar

Abstract

C. dubliniensis was originally isolated from the patients with HIV and recurrent oral candidiasis. C. dubliniensis and C. albicans share many morphological and physiological characters. In the recent scenario C. dubliniensis are known to cause various infections to immunocompromised patients with limited antifungal therapy. In a clinical diagnosis it’s very important to differentiate this agent from other similar Candida spp. A total of 20 clinical isolates of Candida species were obtained from different clinical specimens and subjected to culturing on CHROM agar, Pal’s agar, Casein agar and CHROM with Pal’s agar for the identification of C. dubliniensis. Out of the 20 clinical isolates 8 were found to be C. albicans. Out of these 8 isolates, 4 were observed to produce green colored hyphalfringe colonies in HiChrom agar with Pal’s agar media. This confirms that these were C. dubliniensis. From our study we conclude that CHROM agar supplemented with Pal’s agar is the excellent media for the identification of C. dubliniensis.